Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Muscle
Cell type
Tibialis anterior muscle
NA
NA

Attributes by original data submitter

Sample

source_name
LE2Ex
group
Exercise
tissue
tibialis anterior
genotype
WT
antibody
H3K27Ac

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP assays were performed on powdered tibialis anterior muscle using ChIP-IT High Sensitivity (53040, Active motif, USA) according to the manufacturer's instructions. ChIP-seq libraries were constructed using the Kapa Hyper Prep Kit (KK8500, Roche).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
64727088
Reads aligned (%)
64.0
Duplicates removed (%)
29.9
Number of peaks
45255 (qval < 1E-05)

mm9

Number of total reads
64727088
Reads aligned (%)
64.0
Duplicates removed (%)
29.9
Number of peaks
45307 (qval < 1E-05)

Base call quality data from DBCLS SRA