GSM3754726: CART19 cells, ATAC Nalm6 BID KO Stim rep 3; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CAR-T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Primary human T cells engineered with CD19 CAR
cell type
Primary human T cells engineered with CD19 CAR
treatment condition
15day exposure to BIDko Nalm6
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
cells were processed following the Omni ATAC-seq protocol. To isolate nuclei, cells were washed 2x with ice-cold PBS followed by resuspension in ATAC- resuspension buffer containing 0.1% NP40, 0.1% Tween-20 and 0.01% Digitonin. Cells were incubated on ice for 3 minutes and washed out with ATAC- resuspension buffer containing (0.1% Tween20). Nuclei were pelleted and transposed with Tn5 transposase. nuclei were isolated from 50,000 sorted CART19 cells, followed by the transposition reaction using Tn5 transposase (Illumina) for 30 minutes at 37°C with 1000rp mixing. Purification of transposed DNA was completed with DNA Clean and Concentrator (Zymo) and fragments were barcoded with ATAC-seq indices. Final libraries were double size selected using AMPure beads prior to sequencing.