GSM3752998: H3K27me3 SF1 M 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Embryo
Cell type
Gonadal progenitor cells
NA
NA
Attributes by original data submitter
Sample
source_name
H3K27me3_SF1_M
strain background
C57BL/6 x CD1
genotype/variation
SF1-eGFP
embryonic stage
E10.5
Sex
male
cell type
FACS-sorted gonadal progenitor cells
chip antibody
H3K27me3 CST 9733S
chip protocol
Van Galen et al, 2016
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryonic gonads were micro-dissected at according stage, removed from the mesonephros and incubated in 0.05% trypsin for 5 minutes at 37C. Gonads were then mechanically dissasociated in PBS/3% BSA and passed through a filter cap to obtain a single cell suspension. Fluorescently-labeled cells were collected by Fluorescence Activated Cell Sorting (FACS). For ChIP-seq, 30K-100K cells were collected per replicate. ChIP-seq libraries were prepared with no modifications according to Van Galen et al., 2016.