GSM3747789: Pol II WT Rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse embryonic stem cells
cell type
Embryonic stem cells
treatment
Wild-type
passages
Passage 21
strain
Mouse strain:129 SvJae F1
chip antibody
Pol II (Cell Signaling Technologies, cat# 2629)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For H3K36me3, H3K79me2 and Pol II ChIP-seq experiments: approximately 5x10^7 formaldehyde-crosslinked ESCs were lysed with IP-Star (Diagenode) buffer. Chromatin was sonicated on the Bioruptor (Diagenode) to an average size of 0.2-1 kb. ChIP was performed on chromatin from approximately 5 million cells with 3 μg of antibody (above) using the IP-Star Automated System (Diagenode), and 2.5% of chromatin was used for each whole cell extract (WCE). Following reversal of crosslinks, sample and WCE DNA was purified. ChIP DNA was dissolved in water and placed on the SPRI-TE (Beckman Coulter) for Illumina sample preparation. For H3K9ac, H3K56ac and LEO1 ChIP-seq experiements: approximately 5x10^7 formaldehyde-crosslinked ESCs were lysed and prepared for ChIP-seq according to the protocol described by Black et al., (2013) (PMID: 23871696). For H3K9ac, H3K56ac and LEO1 ChIP-seq experiments: libraries were constructued according to the protocol described by TruSeq ChIP Sample ptreparation from Illumina Part# 15023092 Rev.B, Cat# IP-202-9001DOC.