Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Spermatogonia

MeSH Description

Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.

Sample

source_name

Input_KIT_WT

strain background

C57BL/6

genotype/variation

wild type

age

P7

tissue

testis

cell type

c-Kit+ spermatogonia

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Germ cells were isolated from mouse testis by STA-PUT method or MACS. Cells were fixed in 1% formaldehyde for 10 min at RT. Nuclei were isolated by lysis buffer. Chromatin was sonicated by Covaris sonicater and used for ChIP experiments. see ChIPmentation (PMID: 26280331)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

23001924

Reads aligned (%)

97.9

Duplicates removed (%)

37.1

Number of peaks

291 (qval < 1E-05)

mm9

Number of total reads

23001924

Reads aligned (%)

97.8

Duplicates removed (%)

37.1

Number of peaks

250 (qval < 1E-05)