GSM3738893: H3K79me2 ChIP-seq on 10d cultured HSPC with MLLT3 virus, and treated with EPZ-5676; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K79me2
Cell type
Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
human FL CD34+CD38-/lowCD90+GFP+
cell type
FL-HSPC
chip antibody
Abcam, ab3594
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are crosslinked 10' in 1%formaldehide, lysed and sonicated. The indicated mark is immunoprecipitated overnaight, and enriched by magnetical isolation. IP fraction is eluted and decrosslinked. Ovation Ultralow v2 (Nugen)