GSM3738375: ATAC-seq naive hESC rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC WIBR3
NA
NA
Attributes by original data submitter
Sample
source_name
ATAC-seq_primed_hESC
source
Early Embryo
cell line
WIBR3
culture media
hESM media
status
primed
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-seq was performed as in (Buenrostro et al., 2013) on primed WIRB3 and WIBR3dPE; naïve WIBR3 and WIBR3dPE in 4iLA and KN/2iL media respectively; and in WIBR3dPE in KN/2iL media upon dCAS9-KRAB overexpression containing or not a guide RNA targeting SVA/LTR5Hs Library were made using Nextera DNA Library Prep Kit (Illumina #FC-121-1030) Libraries were ligated with Illumina adaptors Tn5 tagmentation