Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Pre-B cells

NA

NA

Sample

source_name

Adult bone marrow

strain

C57BL/6 x 129/Sv

tissue

bone marrow

cell type

Large transitional pre-B

genotype

WT

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Erythroid, myeloid, T cells, NK cells and mature B lineage (Lin) cells were depleted from BM cell suspensions, and Lin–cKit–/loCD19+CD43+or–CD2– were sorted as large transitional (LTr) pre-B cells by FACS. Nextera DNA sample prep kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

115040298

Reads aligned (%)

95.2

Duplicates removed (%)

48.8

Number of peaks

64616 (qval < 1E-05)

mm9

Number of total reads

115040298

Reads aligned (%)

95.0

Duplicates removed (%)

48.8

Number of peaks

64544 (qval < 1E-05)