Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa
cell line
HeLa
treatment
RNAPII occupancy examined before and after EGF stimulation in starved wild-type Hela Cells
antibody
RNAPII rabbit polyclonal N20 (Santa Cruz, sc-899)
source
IP with Ab RNAPII rabbit polyclonal N20 (Santa Cruz, sc-899)
sample type
ChIP-seq

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq DNA Sample Prep Kit (Illumina, inc.)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
25841739
Reads aligned (%)
97.8
Duplicates removed (%)
6.9
Number of peaks
25052 (qval < 1E-05)

hg19

Number of total reads
25841739
Reads aligned (%)
97.4
Duplicates removed (%)
7.1
Number of peaks
25406 (qval < 1E-05)

Base call quality data from DBCLS SRA