Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Splenic mature B cells
cell type
AID-deficient Splenic mature B cells
passages
0 (primary mature splenic B cells)
strain
C57BL/6
chip antibody
none
treatment
without stimulation

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
NextSeq 550

mm10

Number of total reads
52446658
Reads aligned (%)
98.5
Duplicates removed (%)
21.1
Number of peaks
289 (qval < 1E-05)

mm9

Number of total reads
52446658
Reads aligned (%)
98.5
Duplicates removed (%)
21.4
Number of peaks
276 (qval < 1E-05)

Base call quality data from DBCLS SRA