GSM3734773: 22RV1-DHT-DMSO H3K27Ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
22RV1 immortalized prostate cancer cell line
chip antibody
H3K27Ac (Active Motif, Cat 39133)
cell line
22RV1 immortalized prostate cancer cell line
agent
DMSO + 5 nM DHT, 6 hrs
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated, fixed cell pellets and protein-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation.