Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
22RV1 immortalized prostate cancer cell line
chip antibody
BRD4 (Bethyl, Cat A301-985A100, Lot A301-985A50-6)
cell line
22RV1 immortalized prostate cancer cell line
agent
60 nM ABBV-075 + 5 nM DHT, 6 hrs

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated, fixed cell pellets and protein-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
30506030
Reads aligned (%)
82.4
Duplicates removed (%)
9.4
Number of peaks
2197 (qval < 1E-05)

hg38

Number of total reads
30506030
Reads aligned (%)
85.0
Duplicates removed (%)
7.4
Number of peaks
2551 (qval < 1E-05)

Base call quality data from DBCLS SRA