Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Pro-B cells

NA

NA

Sample

source_name

v-Abl transformed pro-B cell line

cell type

v-Abl transformed pro-B cell line

genotype

JH-del, dCas9 expressing, Sg1-sgRNA expressing, Emu-Bcl2, RAG2 deficient

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked cells were treated with cell lysis buffer (5mM PIPES pH 8, 85mM KCl, 0.5% NP-40) and nuclei lysis buffer (50mM TrisCl pH 8.1, 10mM EDTA, 1% SDS) and subjected to sonication. IP and Input DNA were de-crosslinked at 65o overnight and purified via Qiagen PCR purification columns. Libraries were prepared via the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

69542101

Reads aligned (%)

95.7

Duplicates removed (%)

19.2

Number of peaks

31215 (qval < 1E-05)

mm9

Number of total reads

69542101

Reads aligned (%)

95.5

Duplicates removed (%)

19.5

Number of peaks

31220 (qval < 1E-05)