Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mesenchymal stem cell
cell type
ESC-derived mesenchymal stem cell
genotype
DR8dex2
genotype/variation
transduced with DR8-mtDRBD

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was isolated using phenol-chloroform-isoamylalcohol extraction and ethanol precipitation. The H3K9me3 enriched fragments were contructed into libararies via KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series(KK8504) following the manuals.

Sequencing Platform

instrument_model
HiSeq X Ten

hg38

Number of total reads
16398980
Reads aligned (%)
87.1
Duplicates removed (%)
9.3
Number of peaks
1776 (qval < 1E-05)

hg19

Number of total reads
16398980
Reads aligned (%)
84.3
Duplicates removed (%)
10.5
Number of peaks
485 (qval < 1E-05)

Base call quality data from DBCLS SRA