Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testis

strain

PWD/Ph

age

12 days post partum

genotype

wild-type

tissue

Testis

antibody

N/A

sequencing technique

ChIP-Seq

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated from cells using hypotonic lysis. Chromatin was fragmented by Mnase digestion. After high speed spin soluble chromatin was removed and used for ChIP Libraries were prepared for sequencing using Bioo Scientific's NEXTflex ChIP-Seq Kit (protocol version V11.11) without size selection. Amplification of the libraries was done with 20 µL ligation product, 16 ul water, 12 µL NEXTflex ChIP PCR master mix, 2 µL NEXTflex ChIP primer mix and 14-18 cycles of PCR.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

58319960

Reads aligned (%)

96.5

Duplicates removed (%)

22.9

Number of peaks

278 (qval < 1E-05)

mm9

Number of total reads

58319960

Reads aligned (%)

96.4

Duplicates removed (%)

22.9

Number of peaks

230 (qval < 1E-05)