Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
Treg
NA
NA

Attributes by original data submitter

Sample

source_name
Bcl11b deficient Treg cells
strain background
C57BL/6
genotype/variation
Bcl11b f/f Foxp3-IRES-YFP-Cre
cell type
Sorted CD4+YFP+ cells
molecule subtype
Tagmented DNA

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
100,000 cells from each sample were lysed in hypotonic lysis buffer followed by tagmentaion (enzymatic fragmenting and tagging simultaneously) of DNA. Library from Tagmented DNA were prepared according to manufacturer (Nextera DNA Sample Prep Kit -Illumina) protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
27528517
Reads aligned (%)
76.3
Duplicates removed (%)
16.0
Number of peaks
18355 (qval < 1E-05)

mm9

Number of total reads
27528517
Reads aligned (%)
76.2
Duplicates removed (%)
16.1
Number of peaks
18323 (qval < 1E-05)

Base call quality data from DBCLS SRA