Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Human prostate cell line 22Rv1
tissue
human prostate cancer
treatment
HOXC6 expression
genotype
wild type
cell line
22Rv1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using the DirectZol RNA kit (Zymo, R2062). The RNA_seq libraries were made using Kapa mRNA HyperPrep Kit (KAPA, KKK8581) according to manufacturer's instructions. ChIP_seq libraries were prepared using the Kapa Hyper prep kit (Kapa, KK8503) according to the manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg38

Number of total reads
20476700
Reads aligned (%)
98.8
Duplicates removed (%)
8.5
Number of peaks
17750 (qval < 1E-05)

hg19

Number of total reads
20476700
Reads aligned (%)
98.0
Duplicates removed (%)
8.9
Number of peaks
17774 (qval < 1E-05)

Base call quality data from DBCLS SRA