Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Gonad

Cell type

Ovary

MeSH Description

The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.

Sample

source_name

Ovary, Control – Rep 2

strain

Swiss

treatment

Control

tissue

ovary

chip antibody

H3K4me3 (Merck Millipore, 07-473)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Immunoprecipitated DNA was purified with Qiagen MinElute kit, and 10 µg of DNA was used for library construction. Library was prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

89614423

Reads aligned (%)

98.0

Duplicates removed (%)

21.0

Number of peaks

20148 (qval < 1E-05)

mm9

Number of total reads

89614423

Reads aligned (%)

97.8

Duplicates removed (%)

21.0

Number of peaks

20192 (qval < 1E-05)