Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

actue myeloid luekemia

cell line

MOLM-13

genotype/variation

expressing SIK3T142Q

treatment

2 hr treatment of 0.1 percent DMSO

chip antibody

H3K27ac (abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27ac antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

22094304

Reads aligned (%)

93.4

Duplicates removed (%)

10.9

Number of peaks

36682 (qval < 1E-05)

hg19

Number of total reads

22094304

Reads aligned (%)

93.2

Duplicates removed (%)

11.1

Number of peaks

36789 (qval < 1E-05)