GSM3721808: Line 17 H3.3 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3.3
Cell type
Cell type Class
Neural
Cell type
SU-DIPG-XVII
NA
NA
Attributes by original data submitter
Sample
source_name
Line XVII DIPG
antibody
H3.3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibodies to H3K27me3 (Cell Signaling C36B11) or H3.3 (Millipore #09-838). Libraries were prepared with the Nextera library prep kit Libraries were sequenced on the NovaSeq machine with 150 base paired-end sequencing