Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Gonad

Cell type

Oocytes

MeSH Description

Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Sample

source_name

MII oocytes

genotype

Kdm4a knockout (m-/p-)

cell type

MII oocytes

pooled numbers

300

strain

C57BL/6

chip antibody

H3K9me3 (Pool of Abcam#ab8898, Lot GR153112-1 and Abcam#ab176916)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Qiagene Ultra low input library preperation kit (180492) according to manufacturers instructions and quantified using KAPA library quantification kit from Roche (07960298001)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

45176399

Reads aligned (%)

76.2

Duplicates removed (%)

17.5

Number of peaks

153 (qval < 1E-05)

mm9

Number of total reads

45176399

Reads aligned (%)

76.0

Duplicates removed (%)

17.6

Number of peaks

163 (qval < 1E-05)