Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Gonad

Cell type

A2780

Primary Tissue

Ovary

Tissue Diagnosis

Carcinoma

Sample

source_name

H3K27ac_A2780_CRISPRi_R_1

cell line

A2780-CRISPR/dCas9-KRAB-modified

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein G magnetic beads were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. ChIP-seq libraries were prepared using the KAPA Library Preparation Kit (KK8201)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

18878744

Reads aligned (%)

95.2

Duplicates removed (%)

1.4

Number of peaks

8228 (qval < 1E-05)

hg19

Number of total reads

18878744

Reads aligned (%)

94.5

Duplicates removed (%)

2.4

Number of peaks

8096 (qval < 1E-05)