GSM3720404: H3K27ac A2780 cisplatin; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Gonad
Cell type
A2780
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
H3K27ac_A2780_cisplatin
cell line
A2780
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein G magnetic beads were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. ChIP-seq libraries were prepared using the KAPA Library Preparation Kit (KK8201)