Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Blood

Cell type

MLL-AF9 leukemic cell

NA

NA

Sample

source_name

Mouse primary leukemia

cell type

MLL-AF9 leukemia cells

chip antibody

H3K36me3 (abcam9050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K36Me3, H3K27Me3, H3K9me3, or H3K9me1 antibody. Libraries were prepared using standard NEBNext Ultra I/II DNA Library Prep Kit for Illumina (#E7370S) (#E7645S)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

25783454

Reads aligned (%)

78.0

Duplicates removed (%)

4.6

Number of peaks

155 (qval < 1E-05)

mm9

Number of total reads

25783454

Reads aligned (%)

77.9

Duplicates removed (%)

5.1

Number of peaks

141 (qval < 1E-05)