Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

Jax B6.SJL-CD45.1 CD4+ Th1 cells Input

strain

Jax B6.SJL-PtprcaPepcb/BoyJ (CD45.1)

vendor

The Jackson Laboratory

cell type

CD4+ Th1 cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP assay was performed as described in Oestreich et al. 2014 and Chisolm et al. 2019. Library construction was performed by Genewiz, and samples were subjected to sequencing with paired-end 150bp reads, with the exception of the conventional and germ-free CD8+ Tc1 Rep 1, which was sequenced with single-end 50bp reads.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

59433724

Reads aligned (%)

95.8

Duplicates removed (%)

20.3

Number of peaks

300 (qval < 1E-05)

mm9

Number of total reads

59433724

Reads aligned (%)

95.7

Duplicates removed (%)

20.3

Number of peaks

269 (qval < 1E-05)