Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ubx

Cell type

Cell type Class
Embryo
Cell type
Mesoderm
NA
NA

Attributes by original data submitter

Sample

source_name
mesoderm stage 10-13
genotype/variation
twi-INTACT
developmental stage
mesoderm stage 10-13
cell type
mesoderm
chip antibody
gp-Ubx (home-made)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
11448701
Reads aligned (%)
96.6
Duplicates removed (%)
13.1
Number of peaks
2603 (qval < 1E-05)

dm3

Number of total reads
11448701
Reads aligned (%)
97.4
Duplicates removed (%)
7.6
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA