Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
293_HEK
cell line
293 HEK
antibody
BRD4 (Abcam, 128874)
enrichment target
BRD4
target function
Acetylated Lysine Reader, Active Enhancers
treatment
0nM CEM87, sgMYOD

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. Standard Illumina barcodes were introduced during library preparation.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
35683714
Reads aligned (%)
43.5
Duplicates removed (%)
53.6
Number of peaks
1056 (qval < 1E-05)

hg38

Number of total reads
35683714
Reads aligned (%)
43.9
Duplicates removed (%)
53.3
Number of peaks
1019 (qval < 1E-05)

Base call quality data from DBCLS SRA