Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

H3K4me3_100_ESC

strain

C57BL/6

cell type

native, cultured mESC (V6.5)

chip antibody

H3K4me3, Millipore, 04-745, Lot: 3068440

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ESC was dissociated by 0.1% Trypsin digestion, and aliquot of cell numbers were directly used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

15924835

Reads aligned (%)

7.1

Duplicates removed (%)

55.6

Number of peaks

25 (qval < 1E-05)

mm9

Number of total reads

15924835

Reads aligned (%)

7.1

Duplicates removed (%)

55.5

Number of peaks

22 (qval < 1E-05)