SRX5637700
GSM3711196: H3K27ac E6.5 embryo rep2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Embryo
Cell type
E6.5 embryos
NA
NA

Attributes by original data submitter

Sample

source_name
H3K27ac_E6.5_embryo
strain
C57BL/6
developmental stage
E6.5_embryo
chip antibody
H3K27ac, Abcam, ab4729, Lot: GR3205526-1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
E6.5, E7.0 and E7.75 embryo were directly isolated form uterus and used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
18677613
Reads aligned (%)
57.2
Duplicates removed (%)
59.2
Number of peaks
3286 (qval < 1E-05)

mm9

Number of total reads
18677613
Reads aligned (%)
57.1
Duplicates removed (%)
59.2
Number of peaks
3279 (qval < 1E-05)

Base call quality data from DBCLS SRA