GSM3693094: control H3K4me1 ChIPSeq 4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Lung
Cell type
Lung fibroblasts
NA
NA
Attributes by original data submitter
Sample
source_name
Human_Lung_Fibroblasts_Control
subject status
Control
tissue/cell type
lung fibroblasts
chip antibody
Anti-H3K4me1 (Diagenode, C15410037)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) using a H3K4me1 antibody (Diagenode, C15410037) and the iDeal ChIP-seq kit for Histones kit (Diagenode, C01010051) was performed on the 5 control and 5 IPF fibroblast samples. In addition, we performed ChIP on a single control and IPF using an IgG antibody to provide a background control. Paired-end 75bp sequencing data was obtained using the Illumina HiSeq4000 at the Oxford Genomics Centre at the Wellcome Centre for Human Genetics (funded by Wellcome Trust grant reference 203141/Z/16/Z).