Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Pro-B cells

NA

NA

Sample

source_name

pro-B cell

strain

BALB/c

chip antibody

H3K4me2 monoclonal antibody (Active Motif)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was carried out using the Simple ChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology Inc., Danvers, MA) according to the manufacturer's instructions. SMARTer® ThruPLEX® Tag-seq Kit (Clontech)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

34409985

Reads aligned (%)

96.7

Duplicates removed (%)

11.1

Number of peaks

19570 (qval < 1E-05)

mm9

Number of total reads

34409985

Reads aligned (%)

96.6

Duplicates removed (%)

11.1

Number of peaks

19585 (qval < 1E-05)