GSM3687954: m275 ChIP-seq LNCap -R 87+pGIPZ shFoxA1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Prostate cancer cell line
cell line
LNCaP
genotype
87+pGIPZ shFoxA1
knockdown rna
shFOXA1
transfected with
G87R
treatment
Ethanol
antibody
HA
vendor
Abcam
catalog number
ab9110
lot/batch number
GR261166-6
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin in nuclear fraction were sheared to 200-500bp using an Covaris M220 Focused-ultrasonicator, Lysates were clarified from sonicated nuclei and protein-DNA complexes were immunoprecipitated by using the indicated antibody. DNA was then uncrosslinked from protein, purified and sequenced using Illumina Genome Analyzers followig protocols recommended by the manufacturer. Libraries were prepared according to Illumina's instructions.