At time of collection, cells were trypsinzed, and 70,000 cells (counted by using trypan blue exclusion) were processed for ChIP-sequencing as follows. Cells were fixed with formaldehyde (1%) and reaction was quenched with Glycine 1.25M and Tris 1M pH8. Fixed cells were lysed with SDS lysis solution containing protease inhibitors. Re-suspended pellets were sonicated, precipitated with antibodies (anti-FoxA1 Novus, AR and FLAG) and protein A/G bead complex. The chromatin and immune-complex were sequentially washed with a low-salt solution, high-salt solution, LiCl solution and Tris-NaCl solution. Chromatin was eluted from the complex with a solution containing 1% of SDS and 0.1 mol/l of NaHCO3. Cross-linking between DNA and protein was reversed by adding NaCl solution and incubating at 65°C over-night. Libraries were made using NEBNext Ultra II DNA library prep kit for Illumina (NEB E7645L). Quality control was performed with Bioanalyzer 2200 (Agilent technologies, D1000 screentapes & reagents, cat# 5067-5582) to assess size range of amplified DNA fragments, and with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermofisher cat# P11496) to quantify the DNA fragments generated. ChIP Libraries were then pooled at equimolar concentration and were sequenced multiplexed on the Illumina HiSeq with 50bp paired-end sequencing.