Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Digestive tract

Cell type

Jejunum

MeSH Description

The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.

Sample

source_name

jejunum

strain/background

C57BL/6

genotype/variation

VC+/?Msh2LoxP/LoxP

age

4 months

tissue

jejunum

treatment

none

chip antibody

H3K36me3 (Diagenode, cat. no. C15310058, lot A114-001)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Samples were dissected, shock frozen without delay, and stored in liquid nitrogen until further use. Chromatin was prepared from up to 50 mg tissue using SDS Shearing Buffer and the truChlP Tissue Chromatin Shearing Kit (Covaris, Brighton, UK). ChIPs were run on the IP-Star compact system using the Auto iDeal ChIP-seq kit for histones (Diagenode, Seraing, Belgium). Library preparations were performed according to the TruSeq LT PCR free or TruSeq Nano DNA Kit instructions (Illumina, San Diego, USA). Sequencing was performed on the Illumina HiSeq 4000 with 76 bp paired-end sequencings.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

49420063

Reads aligned (%)

78.9

Duplicates removed (%)

91.0

Number of peaks

125 (qval < 1E-05)

mm9

Number of total reads

49420063

Reads aligned (%)

78.8

Duplicates removed (%)

91.0

Number of peaks

102 (qval < 1E-05)