Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
Mast Cells
MeSH Description
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.

Attributes by original data submitter

Sample

source_name
BDMC_PU.1-ChIP
donr id
donorG
tissue/cell type
breast tissue
cell subtype
breast-derived primary mast cells
chip antibody
PU.1 (Santa Cruz, #sc-352X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq was essentially done as described (Pham et al. 2012). Libraries were generated as described (Pham et al. 2012)

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
38136260
Reads aligned (%)
33.3
Duplicates removed (%)
60.3
Number of peaks
18979 (qval < 1E-05)

hg38

Number of total reads
38136260
Reads aligned (%)
35.1
Duplicates removed (%)
58.6
Number of peaks
18762 (qval < 1E-05)

Base call quality data from DBCLS SRA