Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

Intestinal crypt

NA

NA

Sample

source_name

mouse intestinal Crypt cells

strain

B6; 129 mixed background

cell type

Adult Epitheial cells; proximal 1/3 of intestine

genotype

Eed F/F; Villin-CRE

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysates were sonicated and incubated with H3K27me3 antibody overnight (EMD Millipore 07-449) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 150 bp paired-end reads were sequenced on an NovoSeq S4 instrument.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

38018333

Reads aligned (%)

72.4

Duplicates removed (%)

11.4

Number of peaks

1968 (qval < 1E-05)

mm9

Number of total reads

38018333

Reads aligned (%)

72.3

Duplicates removed (%)

11.4

Number of peaks

1931 (qval < 1E-05)