Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

Intestinal villus

NA

NA

Sample

source_name

mouse intestinal villus cells

strain

B6; 129 mixed background

cell type

Adult Epitheial cells; proximal 1/3 of intestine

genotype

Eed +/+; Villin-CRE

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysates were sonicated and incubated with H3K27me3 antibody overnight (EMD Millipore 07-449) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

36668056

Reads aligned (%)

89.2

Duplicates removed (%)

21.2

Number of peaks

1957 (qval < 1E-05)

mm9

Number of total reads

36668056

Reads aligned (%)

89.0

Duplicates removed (%)

21.2

Number of peaks

1929 (qval < 1E-05)