Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
d5 1mM_Glucose
strain
C57BL/6
tissue
spleens and peripheral lymph nodes
cell type
CD8 T cells
days of glucose restriction
1
genotype
OT-I
chip antibody
anti-H3K27ac (Diagenode, catalog number C15410196)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed cell pellets (1% paraformaldehyde, 10 minutes, RT) were processed for multiplex RELACS (Arrigoni et al., 2018) and sequenced by the staff at the Deep-sequencing Facility at the Max-Planck-Institute for Immunobiology and Epigenetics.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

mm10

Number of total reads
2700522
Reads aligned (%)
82.8
Duplicates removed (%)
18.2
Number of peaks
24 (qval < 1E-05)

mm9

Number of total reads
2700522
Reads aligned (%)
82.7
Duplicates removed (%)
18.2
Number of peaks
25 (qval < 1E-05)

Base call quality data from DBCLS SRA