Antigen

Antigen Class

TFs and others

Antigen

Med1

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver

tissue

liver

genotype

WT

treatment

PTU_T3

chip antibody

MED1 (Santa Cruz, sc-8998)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked sonicated chromatin was IP'ed antibody and protein A/G agarose beads. DNA was extracted by phenol/choloroform. ChIP-seq were constructed using the NEBNext Library prep kit according to manufacturer´s (NEB) instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

29857374

Reads aligned (%)

97.8

Duplicates removed (%)

23.3

Number of peaks

1391 (qval < 1E-05)

mm9

Number of total reads

29857374

Reads aligned (%)

97.5

Duplicates removed (%)

23.3

Number of peaks

1478 (qval < 1E-05)