Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver

tissue

liver

genotype

WT

treatment

PTU

chip antibody

H3K27Ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked sonicated chromatin was IP'ed antibody and protein A/G agarose beads. DNA was extracted by phenol/choloroform. ChIP-seq were constructed using the NEBNext Library prep kit according to manufacturer´s (NEB) instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

11428298

Reads aligned (%)

95.7

Duplicates removed (%)

18.1

Number of peaks

23057 (qval < 1E-05)

mm9

Number of total reads

11428298

Reads aligned (%)

95.6

Duplicates removed (%)

18.2

Number of peaks

23099 (qval < 1E-05)