Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
breast cancer cells
cell line
MCF7
growth protocol
TamR
growth medium
Full media
chip antibody
ERalpha (Santa Cruz, sc-543)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. The extracted ChIP DNA was ligated to specific adaptors using KAPA LTP Library Preparation Kit (KK8230) or KAPA Hyper Prep Kit (KK8504), according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg19

Number of total reads
25877630
Reads aligned (%)
79.3
Duplicates removed (%)
19.6
Number of peaks
42247 (qval < 1E-05)

hg38

Number of total reads
25877630
Reads aligned (%)
80.4
Duplicates removed (%)
18.9
Number of peaks
42304 (qval < 1E-05)

Base call quality data from DBCLS SRA