Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Epidermis

Cell type

A-375

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

melanoma cell line

tissue

skin

cell type

melanoma cells

antibody

CTCF antibody (Cell Signaling Technology; #3418)

cell line

A375

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was sheared to around 150bp to 600bp using Covaris E220 machine and incubated with 6ul CTCF antibody (Cell Signaling Technology; #3418) overnight at 4°C with rotation. ChIP library was constructed using KAPA HTP library prep kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

44844911

Reads aligned (%)

89.7

Duplicates removed (%)

55.7

Number of peaks

8506 (qval < 1E-05)

hg38

Number of total reads

44844911

Reads aligned (%)

90.5

Duplicates removed (%)

54.4

Number of peaks

8672 (qval < 1E-05)