GSM3666054: ChIPSeq WT FO B cells IgG; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Spleen and lymph nodes
NA
NA
Attributes by original data submitter
Sample
source_name
Spleen and lymph nodes
strain
C57BL/6
antibody
rabbit IgG (Santa Cruz)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, and cross-linked with 1% formaldehyde (methanol-free, Pierce, Rockford, IL) at room temperature for 10 min. After sonication, fragmented chromatin equivalent to 10 million cells was immunoprecipitated with control rabbit IgG or indicated antibodies and Magna ChIPTM Protein A+G Magnetic Beads (Millipore, Billerica MA). ChIP-Seq DNA libraries were prepared using KAPA LTP Library Preparation Kit (Kapa Biosystems, Wilmington, MA) and indexed primers (BIOO Scientific, Austin, TX), and the libraries were then sequenced. For RNA-Seq, KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Wilmington, MA), and each library was indexed using barcoded primers (BIOO Scientific, Austin, TX). Barcoded PCR products were purified on 2% E-Gel, 250-400 bp fragments were purified, quantified on Qbit (Invitrogen), mixed, and sequenced on HiSeq2000 platform. PCR products were barcoded (indexed) and sequenced on HiSeq 2000 platform.