Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4 cells

strain

C57BL/6

cell line

G1E ER4

cell type

ES-derived erythroid progenitor cells

treatment

estradiol, nocodazole

mitotic phase

mitosis

chip antibody

Cell Signaling 14958

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's ChIP-seq Sample Preparation Kit. SPRIselect (Beckman Coulter) beads were used obtain an average library target size of 338 bp (range 302-436 bp), followed by real-time qPCR quantification using the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Single-end sequencing (1x50bp or 1x75 bp) was performed on the Illumina NextSeq 500 in high-output mode using Illumina sequencing reagents according to Illumina instructions.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

47388399

Reads aligned (%)

90.0

Duplicates removed (%)

59.8

Number of peaks

446 (qval < 1E-05)

mm9

Number of total reads

47388399

Reads aligned (%)

89.8

Duplicates removed (%)

59.9

Number of peaks

531 (qval < 1E-05)