Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Neural

Cell type

HRPEpiC

Tissue

epithelium

Lineage

ectoderm

Description

retinal pigment epithelial cells

Sample

source_name

Retinal Pigmented Epithelial Cells

tissue

Retinal Pigmented Epithelial Cells

treatment

TNF-alpha + TGF-beta

antibody

H3K4me1 (ab8895, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPs were performed using approximately 10^7 cells fixed with 1% formaldehyde for 10 min at room temp. Sequencing libraries were prepared starting from ~30ng of ChIP DNA with NEBNext Multiplex Oligos for Illumina kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

39691855

Reads aligned (%)

99.3

Duplicates removed (%)

6.4

Number of peaks

53315 (qval < 1E-05)

hg19

Number of total reads

39691855

Reads aligned (%)

99.1

Duplicates removed (%)

6.6

Number of peaks

53308 (qval < 1E-05)