GSM3665505: Input-FBP1 S169A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Liver
Cell type
Hep 3B
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCC
genotype/variation
FBP1 S169A
cell type
Liver cancer cell
passages
10-15
cell line
Hep3B
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody (EZH2 and H3K27me3) or streptavidin beads (FBP1-avi). Illumina sequencing libraries were prepared from ChIP and input DNAs by performing standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation (NEBNext® Ultra™ II DNA Library Prep, NEB, E7103).