Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Hep 3B

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma

Sample

source_name

HCC

genotype/variation

FBP1 S169A

cell type

Liver cancer cell

passages

10-15

cell line

Hep3B

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody (EZH2 and H3K27me3) or streptavidin beads (FBP1-avi). Illumina sequencing libraries were prepared from ChIP and input DNAs by performing standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation (NEBNext® Ultra™ II DNA Library Prep, NEB, E7103).

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

27095172

Reads aligned (%)

97.3

Duplicates removed (%)

11.2

Number of peaks

885 (qval < 1E-05)

hg19

Number of total reads

27095172

Reads aligned (%)

96.6

Duplicates removed (%)

11.4

Number of peaks

445 (qval < 1E-05)