Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Epidermis

Cell type

Dermal fibroblast

NA

NA

Sample

source_name

Adult normal Dermal fibroblast American Type Culture Collection (Catalog Number: PCS-201-012)

tissue

primary dermal fibroblasts

treatment

PGE-2 six hours

cell line

American Type Culture Collection (Catalog Number: PCS-201-012)

chip antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin from formaldehyde-fixed cells (2-5 x 10^6 cells per epitope) was fragmented to a size range of 200 - 700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with the indicated antibodies and Protein G-Dynabeads (Life Technologies). Immunoprecipitated DNA was extracted with AMP Pure beads (Beckman Coulter) after crosslink reversal, RNAse A and Proteinase K treatment. ChIP DNA samples were used to generate Illumina sequencing libraries following standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

40944292

Reads aligned (%)

81.1

Duplicates removed (%)

14.5

Number of peaks

61449 (qval < 1E-05)

hg19

Number of total reads

40944292

Reads aligned (%)

80.8

Duplicates removed (%)

14.6

Number of peaks

61394 (qval < 1E-05)