Antigen

Antigen Class

Histone

Antigen

H4K8ac

Cell type

Cell type Class

Blood

Cell type

Granulocytic-monocytic progenitors

NA

NA

Sample

source_name

KO LGMP cells

strain

C57BL/6

chip-antibody

H4K8ac (#2594, CST, Danvers, MA)

genotype

Prkaa1 and Prkaa2 double knockout

cell type

leukemia-GMP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

50,000 WT or KO L-GMP cells were sorted and treated with Mnase, histone-DNA complexes were isolated with antibody ChIP DNA was purified for end repair, adapter ligation, and amplification using a NEB Ultra II library preparation kit following the manufacturer's protocols (New England BioLabs, Ipswich, MA)

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

3909011

Reads aligned (%)

76.4

Duplicates removed (%)

11.1

Number of peaks

35 (qval < 1E-05)

mm9

Number of total reads

3909011

Reads aligned (%)

76.3

Duplicates removed (%)

11.1

Number of peaks

36 (qval < 1E-05)