GSM3660039: H3K27ac KO LGMP 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Blood
Cell type
Granulocytic-monocytic progenitors
NA
NA
Attributes by original data submitter
Sample
source_name
KO LGMP cells
strain
C57BL/6
chip-antibody
H3K27ac (D5E4, #8173, CST, Danvers, MA)
genotype
Prkaa1 and Prkaa2 double knockout
cell type
leukemia-GMP
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
50,000 WT or KO L-GMP cells were sorted and treated with Mnase, histone-DNA complexes were isolated with antibody ChIP DNA was purified for end repair, adapter ligation, and amplification using a NEB Ultra II library preparation kit following the manufacturer's protocols (New England BioLabs, Ipswich, MA)